Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 JM109 (LMBP 1126)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | JM109 |
| Accession number: | LMBP 1126 |
| Depositor: | This host strain was deposited by Prof. Dr R. Beyaert1 2. It was obtained from Dr J. Messing 3. 1 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium 3 Waksman Institute, Rutgers, State University of New Jersey, Piscataway, USA |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Other culture collection numbers: | DSM 3423 ATCC 53323 NCIMB 12711 |
| Medium: | LB-Lennox |
| Selection marker: | Nalidixic acid |
| Temperature: | 37°C |
| Genotype: | [F´ traD36 proAB laqIqZΔM15] λ- endA1 recA1 gyrA96 thi hsdR17 relA1 supE44 Δ(lac-proAB) |
| Phenotype: | NalR rk- mk+ |
| Properties: | Bacterial Strain JM109 is a useful host for transformation of pGEM Vectors and for production of single-stranded DNA from M13 or phagemid vectors. The strain grows well and is transformed efficiently by a variety of methods. Because JM109 is recA- and lacks the E. coli K restriction system, there is no undesirable restriction of cloned DNA or recombination with host chromosomal DNA. The endonuclease A- mutation leads to an improved yield and quality of isolated plasmid DNA. JM109 is deficient in β-galactosidase activity due to deletions in both genomic and episomal copies of the lacZ gene. The deletion in the episomal (F´ factor) copy of the lacZ gene (lacZΔM15) can be complemented by addition of a functional α-peptide encoded by a pGEM-Z or pGEM-Zf Vector. If complementation does not occur, bacterial colonies are white. To maintain the F´, JM109 should be grown on minimal (M-9) media supplemented with 1mM thiamine. |
| Further information: | There is a genome sequence of the JM109 strain available at Genbank (Accession number: NZ_RYWX00000000.1). The nalidixic acid resistance has been experimentally verified by BCCM/GeneCorner. |
| Original reference: | Yanisch-Perron et al., Gene 33 (1985), 103-119 [PMID: 2985470] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 JM109 (LMBP 1126) is available at BCCM/GeneCorner. It was deposited by Prof. Dr R. Beyaert and was published in Yanisch-Perron et al., 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.